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Reading the Mind of a Fly

New technique could yield knowledge useful to understanding the human brain.

Northwestern University neuroscientists now can read the mind of a fly. They have developed a clever new tool that lights up active conversations between neurons during a behavior or sensory experience, such as smelling a banana. Mapping the pattern of individual neural connections could provide insights into the computational processes that underlie the workings of the human brain.

In a study focused on three of the fruit fly’s sensory systems, the researchers used fluorescent molecules of different colors to tag neurons in the brain to see which connections were active during a sensory experience that happened hours earlier.

Synapses are points of communication where neurons exchange information. The fluorescent labeling technique is the first to allow scientists to identify individual synapses that are active during a complex behavior, such as avoiding heat. Better yet, the fluorescent signal persists for hours after the communication event, allowing researchers to study the brain’s activity after the fact, under a microscope.

“Much of the brain’s computation happens at the level of synapses, where neurons are talking to each other,” said Marco Gallio, who led the study. “Our technique gives us a window of opportunity to see which synapses were engaged in communication during a particular behavior or sensory experience. It is a unique retrospective label.”

Gallio and his team wanted to study the brain activity of a fruit fly while it performed a complex behavior, but this is not easily achieved under a microscope. The scientists figured out a different approach using genetic engineering. Starting with the gene for a green fluorescent protein found in jellyfish, the authors derived three different colored markers that light up at the point of contact between neurons that are active and talking to each other (the synapse). The fluorescent signals can be read one to three hours after the action is over.

“Different synapses are active during different behaviors, and we can see that in the same animal with our three distinct labels,” said Gallio, the paper’s corresponding author.

The fluorescent green, yellow and blue signals enabled the researchers to label different synapses activated by the sensory experience in different colors in the same animal. The fluorescent signals persisted and could later be viewed under a relatively simple microscope.

The researchers studied the fruit fly Drosophila melanogaster, a model animal for learning about the brain and its communication channels. They tested their newly engineered fluorescent molecules by applying them to the neural connections of the most prominent sensory systems in the fly: its sense of smell, sophisticated visual system and highly tuned thermosensory system.

They exposed the animals to different sensory experiences, such as heat or light exposure and smelling bananas or jasmine, to see what was happening in the brain during the experience.

To create the labels, the scientists split a fluorescent molecule in half, one half for the talking neuron and one half for the listening neuron. If those neurons talked to each other when a fly was exposed to the banana smell or heat, the two halves came together and lit up. This only happened at the site of active synaptic transmission.

“Our results show we can detect a specific pattern of activity between neurons in the brain, recording instantaneous exchanges between them as persistent signals that can later be visualized under a microscope,” Gallio said.

This is the kind of new technology scientists discuss in the context of President Obama’s BRAIN (Brain Research Through Advancing Innovative Neurotechnologies) Initiative, Gallio said. Such a tool will help researchers better understand how brain circuits process information, and this knowledge then can be applied to humans.

For the complete article, use this link: http://neurosciencenews.com/genetics-fly-brain-mapping-3219/

It also appeared in The Artificial Intelligencer Dec. 6 Edition: http://paper.li/DaveWolf141/1435187105#!headlines

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I made my bones as an advertising copywriter. My TV, radio and print ads have amused millions of people and helped sell tons of cleaning products, coffee, macadamia nuts and other goodies. But I prefer that other kind of fiction: short stories and novels. My first published novel, Mindclone, is a near-future look at the amusing and serious consequences of brain-uploading. It’s garnered mostly five star reviews. The sequel is percolating in my brain even now. Stay tuned.

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